Phagocytosis Assay of Necroptotic Cells by Cardiac Myofibroblasts

Phagocytosis Assay of Necroptotic Cells by Cardiac Myofibroblasts

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In myocardial infarction slate vcc (MI), a plenty of cardiomyocytes undergo necrosis and necroptosis due to the lack of oxygen and nutrients.The dead cardiomyocytes are promptly engulfed by phagocytes.When the dead cells are not engulfed, the noxious contents of the cells are released outside, and thus, induce inflammation, and obstruct the function of organs.Therefore, phagocytosis is crucial for maintaining homeostasis of organs.Herein, wavertree and london cosmopolitan candle we describe a protocol of an in vitro phagocytosis assay of necroptotic cells.